
ʵÑéÔÀí
ÊÔ¼ÁºÐ½ÓÄÉø½â·¨ÆÆËé½Íĸϸ°û±ÚÀ´ÌáÈ¡½ÍĸÖÊÁ£DNA¡£¡£¡£Ëù½ÓÄɵĽÍÄ¸ÆÆ±ÚøÄÜÓÐÓÃµØÆÆËð½Íĸϸ°û±Ú£¬£¬£¬£¬£¬Ìá¸ß½ÍĸÖÊÁ£DNAµÄ²úÁ¿¡£¡£¡£Îü¸½ÖùÖнÓÄɵĹè»ùÖÊÖÊÁÏÄܸßЧ¡¢×¨Ò»µØÎü¸½DNA£¬£¬£¬£¬£¬¿É×î´óÏÞ¶ÈÈ¥³ýÔÓÖÊ×ðÁú¿Ê±Öʼ°Ï¸°ûÖÐÆäËûÓлú»¯ºÏÎï¡£¡£¡£Ê¹Óñ¾ÊÔ¼ÁºÐÌáÈ¡µÄ½ÍĸÖÊÁ£DNA¿ÉÊÊÓÃÓÚÖÖÖÖͨÀýµÄ·Ö×ÓÉúÎïѧʵÑ飬£¬£¬£¬£¬°üÀ¨Ã¸ÇС¢PCR¡¢²âÐò¡¢ÅþÁ¬ºÍת»¯µÈÊÔÑé¡£¡£¡£
²úÆ·ÓÅÊÆ
1¡¢±¾ÊÔ¼ÁºÐ¿ÉÊÊÓÃÓÚÖÖÖÖ½ÍĸÖÊÁ£µÄÌáÈ¡£¡£¡£¬£¬£¬£¬£¬Äܹ»¿ìËٵĴӽÍĸϸ°ûÖлñµÃ¸ß´¿¶ÈÖÊÁ£¡£¡£¡£
2¡¢ÌáÈ¡µÄ½ÍĸÖÊÁ£DNA¿ÉÓÃÓÚÖÖÖÖͨÀýµÄ·Ö×ÓÉúÎïѧʵÑ飬£¬£¬£¬£¬°üÀ¨Ã¸ÇС¢PCR¡¢²âÐò¡¢ÅþÁ¬ºÍת»¯µÈÊÔÑé¡£¡£¡£
3¡¢±¾ÊÔ¼ÁºÐ²Ù×÷¼òÆÓ¿ìËÙ£¬£¬£¬£¬£¬ÇÒÎÞÐèʹÓ÷ӡ¢ÂȷµÈÓж¾ÊÔ¼Á¡£¡£¡£
ÊÔ¼ÁºÐ×é·Ö
| ×é·Ö | ÈÝÁ¿£¨100T£© | ×é·Ö | ÈÝÁ¿£¨100T£© |
| RNase A | 500μL | ÒºYP3 | 40mL |
| ÓÎÄ¸ÆÆ±Úø | 1.4mLx2 | ƯϴҺ | 30mL |
| ÆÆ±ÚBuffer | 50mL | Ï´ÍÑÒº | 10mL |
| ÛÏ»ù»¹Ô¼Á | 800μL | Îü¸½Öù | 100¸ö |
| Ó£ÒºYP1 | 30mL | ÍøÂç¹Ü | 100¸ö |
| º£ÒºYP2 | 30mL | ˵Ã÷Êé | 1·Ý |
×¢ÖØ£ºÊ¹ÓÃǰÇëÏÈÔÚ30mLƯϴҺÖмÓÈë60mLÎÞË®ÒÒ´¼¡£¡£¡£ÈÜÒºYP1ÔÚʹ?ǰÏȼÓÈëRNaseA£¨½«ÊÔ¼ÁºÐÖÐÌṩµÄ RNaseA ËùÓмÓÈ룩£¬£¬£¬£¬£¬»ìÔÈ£¬£¬£¬£¬£¬ÖÃÓÚ2-8¡æÉúÑÄ¡£¡£¡£
³£¼ûÎÊÌâÓë½â´ð
Q1: ¹ØÓÚ½ÍĸÖÊÁ£Ð¡ÌáÊÔ¼ÁºÐÓÐûÓжÔÓ¦µÄÎÄÏ×
²Î¿¼ÎÄÏ×£ºIdentification of Transcription Factors of Santalene Synthase Gene Promoters and SaSSY Cis-Elements through Yeast One-Hybrid Screening in Santalum album L. ÔÚÎÄÕÂÖÊÁϺÍÒªÁìÖÐÌáµ½¹«Ë¾£¬£¬£¬£¬£¬ÎÄÕÂÁ´½Ó£ºhttps://www.mdpi.com/2223-7747/13/13/1882
Éó²é¸ü¶àÎÄÏ×£ºÊ×Ò³-ÎÄÏ×-¶ÔÓ¦ÅÌÎʼ´¿ÉÕÒµ½¡£¡£¡£ÍøÖ·£º/citations/
Ïà¹Ø²úÆ·&¼¼ÊõЧÀÍ
Ïà¹Ø²úÆ·
⇒ ÒøÈ¾ÊÔ¼ÁºÐ(Silver Stain Kit) ⇒ Co-IPÊÔ¼ÁºÐ£¨Ö²Î
⇒ ChIPÊÔ¼ÁºÐ£¨¶¯Î ⇒ ChIPÊÔ¼ÁºÐ£¨Ö²Î
⇒ RIPÊÔ¼ÁºÐ ⇒ RNA pull-downÊÔ¼ÁºÐ
⇒ DNA pull-downÊÔ¼ÁºÐ£¨¶¯Î ⇒ DNA pull-downÊÔ¼ÁºÐ£¨Ö²Î
Ïà¹Ø¼¼ÊõЧÀÍ
? ˫ӫ¹âËØÃ¸±¨¸æÏµÍ³ ? ½Íĸ˫ÔÓ½» ? EMSA ? RNA pull-down
? RIP-qPCR ? DNA pull-down ? ChIP-qPCR ? ½Íĸµ¥ÔÓ½»
×ðÁú¿Ê±

