×ðÁú¿Ê±
ΪʲôÖÚ¶à¿ÆÑÐְԱѡÔñ½ð¿ªÈðµÄCo-IP¼ì²âЧÀÍ
½ÓÄÉÌØÒìÐÔ¶þ¿¹¾ÙÐмì²â£¬£¬£¬£¬£¬ÓÐÓÃ×èÖ¹¿¹ÌåÖØÁ´¶ÔЧ¹ûµÄÐźÅ×ÌÈÅ
ÓÅ»¯µÄʵÑéÌõ¼þºÍ¼¼ÊõÁ÷³Ì£¬£¬£¬£¬£¬È·±£¼ì²âЧ¹ûµÄѸËٶȺͿɿ¿ÐÔ
ÿһ²½ÊµÑé¶¼¾ÓÉÑÏ¿áµÄÖÊÁ¿¿ØÖÆ£¬£¬£¬£¬£¬È·±£Êý¾ÝµÄ׼ȷÐԺͿÉÖØ¸´ÐÔ
±ê×¼Á÷³ÌÓÅ»¯£¬£¬£¬£¬£¬Ëõ¶ÌʵÑéÖÜÆÚ£¬£¬£¬£¬£¬¿ìËÙ½»¸¶¸ßÖÊÁ¿Ð§¹û
ÃâÒß¹²³Áµí¼¼Êõ£¨Co-Immunoprecipitation, Co-IP£©ÊÇÑо¿Á½ÖÖ×ðÁú¿Ê±ÔÚÌåÄÚÊÇ·ñ±£´æÏ໥×÷ÓõÄÓÐÓÃÒªÁì¡£¡£¡£¡£
ͨ¹ý¿¹ÌåºÍÒÑÖª×ðÁú¿Ê±Á¬Ïµ£¬£¬£¬£¬£¬´Ó¶ø²¶»ñÕû¸öÒÑÖª×ðÁú¿Ê±¸´ºÏÎ£¬£¬£¬£¬½ø¶øÑо¿¸´ºÏÎïÖÐÓëÒÑÖª×ðÁú¿Ê±±£´æÏ໥×÷ÓõÄ×ðÁú¿Ê±¡£¡£¡£¡£
½ð¿ªÈðÌṩרҵµÄCo-IP¼ì²âЧÀÍ£¬£¬£¬£¬£¬Ë¢Ð¼¼ÊõʹÓÃÌØÒìÐÔ¶þ¿¹¾ÙÐзÖ×Ó»¥×÷¼ì²â£¬£¬£¬£¬£¬ÄÜÓÐÓÃ×èÖ¹¿¹ÌåÖØÁ´¶ÔЧ¹ûµÄÐźÅ×ÌÈÅ¡£¡£¡£¡£
ÎÒÃÇÔÊÐí£ºÈôʵÑéЧ¹ûδµÖ´ïÌõÔ¼ÒªÇ󣬣¬£¬£¬£¬¿ÉÌṩÍêÉÆµÄÊÛºóЧÀÍ
±ê×¼»¯Á÷³ÌÈ·±£ÊµÑéЧ¹û׼ȷ¿É¿¿
¿Í»§Ìá½»ÑùÆ·¼°Ïà¹ØÐÅÏ¢£¬£¬£¬£¬£¬ÎÒÃǾÙÐÐÑùÆ·ÑéÊպ͹ҺÅ
ÖÆ±¸ÑùÆ·ÁѽâÒº£¬£¬£¬£¬£¬²¢¾ÙÐÐ×ðÁú¿Ê±¶¨Á¿£¬£¬£¬£¬£¬È·±£ÊµÑéÖÊÁÏÇкÏÒªÇó
½«ÑùÆ·Ó뿹Ìå¼°protein A/GÄý½º¾ÙÐзõÓý£¬£¬£¬£¬£¬ÐγÉÃâÒ߸´ºÏÎï
ÍøÂçÃâÒß³ÁµíÎ£¬£¬£¬£¬¾ÙÐÐÏ´µÓÈ¥³ý·ÇÌØÒìÐÔÁ¬Ïµ£¬£¬£¬£¬£¬ÖƱ¸ÑùÆ·
ͨ¹ýWestern Blot¼ì²âÄ¿µÄ×ðÁú¿Ê±¼°Ï໥×÷ÓÃ×ðÁú¿Ê±
ÌṩÍêÕûµÄʵÑ鱨¸æºÍÊý¾Ý½â¶Á£¬£¬£¬£¬£¬È·±£¿£¿£¿£¿£¿Í»§»ñµÃÓмÛÖµµÄЧ¹û
Ϊȷ±£ÊµÑé˳Ëì¾ÙÐУ¬£¬£¬£¬£¬ÇëÌṩÒÔÏÂÖÊÁÏ
| ЧÀÍÃû³Æ | ЧÀÍÄÚÈÝ | ½»¸¶ÄÚÈݼ°±ê×¼ | ЧÀÍÖÜÆÚ(ÊÂÇéÈÕ) |
|---|---|---|---|
| ÃâÒß¹²³ÁµíCo-IP | ×ðÁú¿Ê±ÌáÈ¡ | 1¸öÑù±¾ | 16 |
| Co-IPǰwb | Åܽº1¸öÓ¾µÀ | ||
| Co-IP | ¸»¼¯2´Î£ºIgG¡¢IP¸÷1´Î | ||
| Co-IPºóÓÕ¶ü×ðÁú¿Ê±wb | Åܽº3¸öÓ¾µÀ£ºIgG¡¢IP¡¢input | ||
| SDS-PAGEÒøÈ¾ | Åܽº3¸öÓ¾µÀ£ºIgG¡¢IP¡¢input | 4 | |
| ÖÊÆ×Åжϻ¥×÷×ðÁú¿Ê± | ÖÊÆ×ÅжϣºIgG¡¢IP | 10-15 |
ÖÚ¶àÀֳɰ¸Àý£¬£¬£¬£¬£¬ÑéÖ¤×ðÁú¿Ê±×¨ÒµÄÜÁ¦
ͨ¹ýCo-IP¼¼ÊõÀÖ³ÉÑéÖ¤Á½ÖÖ×ðÁú¿Ê±ÔÚÌåÄÚµÄÏ໥×÷Ó㬣¬£¬£¬£¬Ð§¹ûΪºóÐø¹¦Ð§Ñо¿ÌṩÓÐÁ¦Ö¤¾Ý¡£¡£¡£¡£
£¨ÎÄÏ×£ºAberrant oligodendroglial LDL receptor orchestrates demyelination in chronic cerebral ischemia. ÆÚ¿¯£ºJ Clin Invest IF£º13.6£©
ʹÓÃCo-IPÁ¬ÏµÖÊÆ×¼¼Êõ£¬£¬£¬£¬£¬ÀÖ³ÉÅжÏÄ¿µÄ×ðÁú¿Ê±µÄÏ໥×÷ÓÃÍøÂ磬£¬£¬£¬£¬Õ¹ÏÖеÄÉúÎïѧ¹¦Ð§¡£¡£¡£¡£
£¨ÎÄÏ×£ºPFN1 Inhibits Myogenesis of Bovine Myoblast Cells via Cdc42-PAK/JNK. ÆÚ¿¯£ºCells IF£º5.2£©
ÃâÒß¹²³Áµí£¨Co-IP£©¼¼ÊõÖ÷ÒªÓÃÓÚ£º
CoIPʵÑéÐèÒª°ü¹Ü×ã¹»µÄ×ðÁú¿Ê±Å¨¶È²Å»ªÎ¬³ÖÔ±¾±£´æµÄ×ðÁú¿Ê±»¥×÷״̬£¬£¬£¬£¬£¬ÓÈÆäµ±×ðÁú¿Ê±Æ·Ã²½ÏµÍ¡¢Ï໥×÷ÓÃÁ¦²»Ç¿¡¢Ê¹Óò»Ò×Ìá×ðÁú¿Ê±×éÖ¯µÈÇéÐÎʱ¡£¡£¡£¡£²¢ÇÒCoIPµÄʵÑéÌõ¼þÍùÍùÐèÒªÖØ¸´Ì½Ë÷¡£¡£¡£¡£Òò´ËÓÃÓÚCoIPʵÑéÐè×¼±¸Ï¸°û£¾2x10^7£¬£¬£¬£¬£¬¶¯Îï×éÖ¯£¾500mg£¬£¬£¬£¬£¬Ö²Îï×éÖ¯£¾2g£¬£¬£¬£¬£¬²¢ÇÒÐëÔ½·¢×¢ÖØÊÕÂÞºóËÙ¶³-80¡æÉúÑĺÍ×èÖ¹ÖØ¸´¶³ÈÚ¡£¡£¡£¡£
a.ËùʹÓõĿ¹Ìå²»µ«ÐèÒªÓÅÒìµÄÇ׺ÍÁ¦¡¢ÌØÒìÐÔ£¬£¬£¬£¬£¬Æäʶ±ð±íλ»¹¿ÉÄܱ»»¥×÷×ðÁú¿Ê±ËùÕڱΣ¨Ö÷ÒªÊÇʹÓõ¥¿¹Ê±£©£»£»£»
b.µ±×ðÁú¿Ê±»¥×÷Ð뱬·¢ÔÚÌØ¶¨ÐÄÀí´úлÌõ¼þ¡¢×é֯ϸ°ûÀàÐÍÖ®ÖÐʱ£¬£¬£¬£¬£¬CoIPʵÑé¿ÉÄÜÎÞ·¨»ñµÃ»¥×÷Ч¹û£»£»£»
c.ÈôÊÇÓÕ¶ü×ðÁú¿Ê±ºÍ»ò²¶»ñ×ðÁú¿Ê±ÔÚÑù±¾ÖÐÆ·Ã²ºÜµÍʱ£»£»£» d.Á½¸ö»¥×÷×ðÁú¿Ê±µÄÇ׺ÍÁ¦½ÏÈõ£»£»£»
e.µ±¸Ã×ðÁú¿Ê±»¥×÷ÐèÒª½ÏÌØ¶¨µÄÀë×ÓÇ¿¶È£¬£¬£¬£¬£¬»òÕßÐèÒªÈçCa2+Àë×Ó/Mg2+Àë×Ó/ATPµÈ¸¨Òò×Óʱ£¬£¬£¬£¬£¬Òª´´Á¢ºÏÊʵķ´Ó¦Ìõ¼þ»á±äµÃÄÑÌ⣻£»£»
f.һЩÑùÆ·´¦Öóͷ£ÌáÈ¡±£´æÄѶȣ¬£¬£¬£¬£¬ÌáÈ¡×ãÁ¿Ä¿µÄ×ðÁú¿Ê±Óë¼á³Ö×ðÁú¿Ê±×ÔȻ״̬£¬£¬£¬£¬£¬Ðèͨ¹ýʵÑéÌõ¼þµÖ´ïÓÅ»¯Æ½ºâ£»£»£»
g.ÍâÔ´±í´ïµÄ±êÇ©×ðÁú¿Ê±±£´æÍ¬ÄÚÔ´×ðÁú¿Ê±µÄλµã¾ºÕù£¬£¬£¬£¬£¬ÕâÖ÷ÒªÔÚʹÓñêÇ©¿¹Ìå¾ÙÐÐʵÑéʱ¿ÉÄܱ£´æ¡£¡£¡£¡£
ÔÚCoIP-WBÅжÏЧ¹ûÖУ¬£¬£¬£¬£¬IgG×éÎÞÄ¿µÄ×ðÁú¿Ê±Ìõ´ø¡¢IP×éÓÐÄ¿µÄ×ðÁú¿Ê±Ìõ´ø£¬£¬£¬£¬£¬ËµÃ÷IPÀú³ÌÀֳɣ»£»£»ÒøÈ¾½ºÍ¼ÖУ¬£¬£¬£¬£¬IP×éÏà¹ØÓÚIgG×éÓиü¶à×ðÁú¿Ê±Ìõ´ø¡¢ÇÒ±£´æ²î±ð£¬£¬£¬£¬£¬ËµÃ÷CoIPÀú³Ì²¶»ñµ½»¥×÷×ðÁú¿Ê±£»£»£»IP²úÆ·ÔÚÖÊÆ×¼ì²âÖÐÅжϵ½µÄ×ðÁú¿Ê±ÊýÄ¿²»¿É×÷ΪÅжÏʵÑé³É°ÜµÄ±ê×¼£¬£¬£¬£¬£¬ÓÉÓÚÕâÈ¡¾öÓÚÄ¿µÄ×ðÁú¿Ê±×Ô¼ºËù¾ßÓеĻ¥×÷×ðÁú¿Ê±ÊýÄ¿¡¢½áÐÁ¦Ç¿ÈõµÈ·½Ã棻£»£»IP²úÆ·µÄÖÊÆ×ÅжÏÊýĿԽ¶à²¢²»´ú±íЧ¹ûÔ½ºÃ¡£¡£¡£¡£
Ìá½»ÄúµÄÏîÄ¿ÐèÇ󣬣¬£¬£¬£¬×ðÁú¿Ê±¼¼Êõר¼Ò½«ÔÚ24СʱÄÚÓëÄúÁªÏµ£¬£¬£¬£¬£¬Ìṩרҵ½â¾ö¼Æ»®
ЧÀÍʱ¼ä£ºÖÜÒ»ÖÁÖÜÎå 9:00-18:00
ÐëÖª
Ê×´Î΢ÐÅɨÂëÓû§°ì·¨£º
1.΢ÐÅɨÂë¡£¡£¡£¡£
2.±¾Ò³ÃæÍêÉÆÊÖ»úÑéÖ¤¡£¡£¡£¡£
ɨÂë×Éѯ
µç»°£º027-87960366